Entering edit mode
8.1 years ago
gwilson9
•
0
I have three replicate paired-end RNA-seq samples that I would like to run through Tophat. I submitted the runs through command line with this code (generic):
tophat -p # genome_index read1_fwd,read2_fwd,read3_fwd read1_rev,read2_rev,read3_rev
and received this error message: Pair #1 name mismatch: read1_fwd vs read2_fwd.
I'm new to this, but it looks like the software thinks that two samples in the first group are intended pairs. Is there a better way that I should submit this job? Thanks for your help!
It's likely that you put a space somewhere where you shouldn't.
BTW, unless the replicates are technical you shouldn't align them this way.
Thanks, you called it. The samples are actually biological replicates. Should they be run separately and merged after aligning?
RNA-seq triple replicates