NCBI urlapi parameter setting

Question

[morteza.mahmoudisaber]

I want to automate some blast searches, I read NCBI URLAPI TUTORIAL but I couldn't find any explanation about two issues

for example in the setting the parameter values:

http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn

1. How can I check a Exclude checkbox for organism?
2. How can I insert query by uploading a FASTA text file from my computer?

I appreciate any hints

REGARDS

[Pierre Lindenbaum]

have a look at How To Build The Correct Blast Url For The Rest Interface for an example.

[morteza.mahmoudisaber]

Thanks for link.

But in the code there, you inserted sequence directly, not by uploading a file and you didnt used the organism Exclude checkbox.

I found this hint in NCBI webpage:

https://www.ncbi.nlm.nih.gov/staff/tao/URLAPI/new/node58.html#sub:QUERY

it says like the command line, the -i should be used to upload a file, but I dont know how to do it in the url and unfortunately it didn't give any examples

do you know how it should be used?