Rna Fragmentation Technique Has A Significant Effect On Transcript Reassembly

Question

[support]

Interesting paper examines transcriptome reassembly and determines it is sensitive to RNA fragmentation technique. Concludes that Zn mediated fragmentation provides a better transcript ID compared to cleavage by RNase III: http://www.sciencedirect.com/science/article/pii/S1046202313000789

I wonder if this has been tested with Mg. Other methods to fragment nucleic acid: http://blog.genohub.com/fragmentation-of-dna-rna-for-next-gen-sequencing-library-prep/

-- Genohub

Answer 1

[madkitty]

No idea, though it could be an interesting BS/MS project if anyone has enough funding for that :)