Mutation protein program at the exactly point

Question

[ganuongphap]

Based on this How To Generate Mutations In Sequences Of A Fasta File?, I found EMBOSS program which helped to mutated a protein. However, this tool only mutates randomly. I would like to have a tool or a script which help me mutate at a specific point in the protein. Can you list some for me? Thank you.

[Bernard Offmann]

Hi,

check my scripts at : http://bo-protscience.fr/peaccel/Mutagenesis.html

Bernard

[ganuongphap]

Your tool is great! Is it possible to download it?

[Devon Ryan]

That's easy enough to do by hand that I doubt anyone has bothered. You could also trivially do that with biopython or bioperl.

Answer 1

[vlaufer]

Do you mean a tool that would predict the effect of a mutated site?

If so, are you referring to structural implications, or just predicted deleteriousness?

If you just want to check whether a given variant is likely to be damaging? If you mean that, you might try CADD: http://cadd.gs.washington.edu/

Hope that helps.

[ganuongphap]

After looking its website, I think this tool is for gene mutation, not protein mutation. I'm studying in protein, not gene.