Question

Bowtie2 for 454 Data?

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10.5 years ago

zhwjmch ▴ 170

The DNA data was produced by MWG-Biotech: 454 (GS FLX machine) sequencing by emulsion PCR.

Is Bowtie2 a good choice for mapping those reads to the whole human genome? Any other suggestions?

Thanks in advance.

alignment • 2.2k views

ADD COMMENT • link updated 10.5 years ago by dariober 15k • written 10.5 years ago by zhwjmch ▴ 170

Ram · Answer 1 · 2014-05-30

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10.5 years ago

zhwjmch ▴ 170

"It handles very long reads (i.e. upwards of 10s or 100s of kilobases), but it is optimized for the read lengths and error modes yielded by recent sequencers, such as the Illumina HiSeq 2000, Roche 454, and Ion Torrent instruments."

cited from Bowtie2 online manual

ADD COMMENT • link updated 5.1 years ago by Ram 44k • written 10.5 years ago by zhwjmch ▴ 170

Ram · Answer 2 · 2014-05-30

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10.5 years ago

dariober 15k

What about bwa mem?

From the FAQ:

For 70bp or longer Illumina, 454, Ion Torrent and Sanger reads, assembly contigs and BAC sequences, BWA-MEM is usually the preferred algorithm.

ADD COMMENT • link updated 5.1 years ago by Ram 44k • written 10.5 years ago by dariober 15k