Entering edit mode
10.6 years ago
arronar
▴
290
Hello!
I would like to ask you if its possible to run a tblastx (Search translated nucleotide database using a translated nucleotide query) or blastx (Search protein database using a translated nucleotide query) against some SRA files locally. SRA files will be the database in this situation. I saw that NCBI supports only blastn against SRA databases.
Thank you.
I think you need to convert the .SRA files to either fastq or fasta before performing the analysis.