Real Time Experiment Statistical Analysis

Question

[Ginger]

hi guys I am really new to statistics and I need your help. I have done a routine real time experiment. I have ct, delta delta ct, RQ related to untreated sample. What should i do with these data??? I like to have graph of results like those seen in research articles having error bars. How should I do this? I do not like graphs produced in excel. I'll be grateful to have ur suggestions

Answer 1

[Neilfws]

If you're comfortable with (or willing to learn) R-based solutions, the ddCt package in Bioconductor is worth a look.

Answer 2

[Chris Evelo]

You could search Biostar for "PCR" and especially check this question: Software For Analysing Real-Time Pcr Data

I think your answer will already be there.

Answer 3

[Darren J. Fitzpatrick]

Depending on the nature of your project - is it an undergraduate dissertation, Masters or Ph.D.?

If any of Masters or Ph.D. I recommend putting the time in to learn R. It is a great tool and once you master those finicky apply functions - the world is your oyster. Well, I might exaggerate a little.

The plots you are looking for are boxplots (the ones with the error bars). Launch R and type:

?boxplot

at the prompt in order to get a description of the function and it's usage. Also, a great resource to learn basic R very quickly is:

http://www.statmethods.net/

Good Luck!

[A.L]

Some nice R examples are available here : http://monkeysuncle.stanford.edu/?p=485