Accession Codes for Ganoderma lucidum

Question

[evaemee28]

Currently I am working on my final year project. I have choose to study ganoderma lucidum.

I need to choose a good accession number(NCBI) in order to obtain good sequence that will help to find non ribosomal peptidase. What are the criteria that I should see when choosing a proper accession number?

When I tried to see to see the difference but the only difference I see is the technology used which Illumina and Roche 454 and also the other difference is in Library: Layout which stated Single or Paired, Nominal length.. I dont understand this either.

Please help

Answer 1

[Josh Herr]

What exactly are you actually looking for? Yes, a non-ribosomal peptidase, but what are you looking for and where are you looking? Criteria? -- A full length sequence would suffice. This all depends on what you plan to do with the sequence.

Please put some time into addressing your knowledge about basic sequencing - there are many many resources available on the different types of sequencing technologies and the differing sequencing techniques such as single end or paired end.

Two Ganoderma lucidum genomes have been sequenced (here and here). JGI provides genome data for another strain here</a>. That is three genomes for you to work with. Best of luck.

[evaemee28]

Noted for the basic sequencing.

Actually I found some accession numbers to download the sequence for ganoderma lucidum in NCBI to find nonribosomal peptiase in ganoderma lucidum, but there are many accession numbers for ganoderma lucidum. I am confused which I am suppose to choose.