How to use R package limma to find specific gene?

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10.6 years ago

zero_hsy ▴ 110

Hello,I want to find whether this gene is specific or not.What I want to use is the R package limma.

For example,this gene A has two conditions. X(disease):x1,x2,x3 Y(normal):y1,y2,y3,y4,y5,y6. How can I use the limma to calculate the p-value like t-test?

R package limma specific gene • 2.9k views

ADD COMMENT • link updated 3.2 years ago by Ram 44k • written 10.6 years ago by zero_hsy ▴ 110

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If this isn't microarray/RNAseq or similar data where you have more than one gene being measured at a time then you'll get no benefit from using limma vs. just doing a standard T-test.

ADD REPLY • link 10.6 years ago by Devon Ryan 105k

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Is this microarray data? Limma should work just fine if that is the case. What problems did you have when you ran limma?

ADD REPLY • link updated 3.2 years ago by Ram 44k • written 10.6 years ago by Sean Davis 27k

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