Currently I am predicting miRNA in animal.. when I perform BLASTx, i dont know how to select the non-coding protein? plz help me.. Do we have any other softwares or tools to remove coding protein??
Sorry but this question is confusing on so many levels that it's barely understandable.
Predicting miRNAs.. the precursors? Or do you mean miRNA targets, and how exactly are you trying to predict them?
There's no such thing as non-coding or coding protein. Do you mean non-coding transcripts?
Blastx works on the assumtion that you try to map coding mRNA to a protein database. What would be the purpose of removing proteins in that context? What are you trying to achieve?
There's no such thing as non-coding or coding protein. Do you mean non-coding transcripts? I think it's OK
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version 2.3.6
ya.. First of all I am trying to predict the precursors and then the miRNA target. I dont have much knowledge in this area of prediction. I am new to this and I read many papers such as miRNA prediction using EST like that..
I am following the miRNA prediction using EST. what exactly I want means I want to retain the non-protein coding sequences from the blastn result against the EST of particular animal.. Because miRNA is the non-protein-coding small RNAs.