Question

Is size selection always necessary in building NGS library?

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10.7 years ago

epigene ▴ 590

I've seen NGS library protocols always have a size selection step after adapter ligation and amplification. I'm wondering why is size selection needed and what does it do exactly to help the sequencing? Is it always necessary to do it?

Thanks!

sequencing • 2.2k views

ADD COMMENT • link updated 3.4 years ago by Ram 45k • written 10.7 years ago by epigene ▴ 590

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What purpose are these reads being used for?

ADD REPLY • link 10.7 years ago by Zev.Kronenberg 12k

score 1 · Answer 1 · 2014-08-13

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10.7 years ago

Istvan Albert 102k

The sequence sizes need to fall within a certain range for the instrument to be able to sequence them.

ADD COMMENT • link 10.7 years ago by Istvan Albert 102k

score 1 · Answer 2 · 2014-08-13

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10.7 years ago

Asaf 10k

Another reason is removing primer dimers

ADD COMMENT • link 10.7 years ago by Asaf 10k