how can identify read depth

Question

[hana]

Hi

I would like to check the read depth of my row sequences received from company.

Any suggestion would be appreciated

Thank you

Answer 1

[Biomonika (Noolean)]

Get number of reads, multiply it by read length, divide by size of your genome. If you have RNASeq, you need to know the estimate of the transcribed proportion of the genome.

[hana]

Thank you so much for your reply. I have the RNASeq from human. Would you please let me know what is the human transcriptome size?

Thank you