Hi all,
I've just received a matrix of RNA_seq data (genes at the rows, samples at the columns and values at each cell), where the values at the cells are median-centered values. I would like to know if it's possible to perform a differential expression analysis using limma with this data.
Thanks in advance,
Joan
It'd be simpler if whomever gave you the dataset could simply provide you with either the raw or estimated counts. Then you don't have to monkey around with things as much.