Entering edit mode
10.2 years ago
sfcarroll
▴
80
In the Primer 3 manual, it details where fasta format mispriming libraries can be found on the internet, eg:
The documentation for mispriming libraries can be found here
Is the correct way to use these libraries like this:
- Download the mispriming libraries, and save them as .fa, eg: human.fa
- When running Primer 3 from the command line, pass the appropriate mispriming library path.
e.g.:
PRIMER_MISPRIMING_LIBRARY='/home/primer3/mispriming/human.fa'
Is this correct?