trinity assemble error , shouldn't have negative delta during EM: -1.5625, round: 84

Question

[wangjun19820303]

I run the trinity

./Trinity --seqType fq --JM 32G --left /data2/trinity/clean_data/Sf9-all_1.fq --right /data2/trinity/clean_data/Sf9-all_2.fq --CPU 18 --out Sf9_all_assemble

The assembly does not complete.The problem like this in Butterfly:

butterfly_commands
-shuffle -CPU 24 -failed_cmds failed_butterfly_commands.35911.txt -v *
*Number of  Commands: 105304*
*succeeded(65294)   62.0052% completed.   shouldn't have negative delta during EM: -1.5625, round: 84

Error is same as in this email : sourceforge.net/p/trinityrnaseq/mailman/message/32792538/

so I download the newest Butterfly.jar ,but the error is still in there.

Thanks in advance for your help.

[Coryza]

Have you considered e-mailing the author of Trinity?

b h a a s @ b r o a d i n s t i t u t e . o r g

[wangjun19820303]

I will try,thank you.

[Coryza]

And? Any updates?

[Biojl]

Why does it try to use 24 cpu if you specify 18 in the command?

[wangjun19820303]

Sorry, I paste the error parameter of cpu,they were both 18.