Calculate Genotype quality from the PL
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10.2 years ago
stat.1405 ▴ 30

I have an output of the call variants, VCF format.

I do not have the genotype quality (GQ) which is in the Format section. I have just the likelihood of the genotype (PL).

So, How can I calculate the genotype given the likelihood PL.

for example GT:PL:DP 1/0:255,0,255:62 how can I get the GQ?

Thanks

next-gen alignment SNP • 4.6k views
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10.2 years ago

unphred(0) / (unphred(0) + unphred(255) + unphred(255)). You example isn't ideal as it is pretty as perfect as a call gets.

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Good, changing from phred score to unphred is 10^(-Q/10)

10^(-0/10)/10^(-0/10)+10^(-255/10)+10^(-255/10) = GQ is this correct

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Missing parentheses

fraction(best score)(sum of unphred all scores)

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