molecular weight of the truncated protein using a 2nd AUG downstream of the normal AUG

Question

[datanerd]

Hi all,

We have a protein where the mutation is affecting the start codon. Could someone please direct me to a tool that would help determins the second (AUG) start codon and the estimated weight of the protein.

Thanks!!
Mamta

Answer 1

[Whetting]

you mean this? http://web.expasy.org/compute_pi/

[datanerd]

Thanks Whetting! This is helpful!

This is useful when I get the new sequence for the protein with the potential new start codon. How do I get the next possible start codon for the gene if the original is mutated?

Mamta

[Whetting]

you can just look for the first in frame ATG? Alternatively, check out ORFfinder at NCBI