When analysing transcrptomics or metagenomics of two or more samples, there may be many contigs similar with each other. How can I regard those contigs as a same gene fragment? which index or parameter can be employed? Is there any high effective analysis tool to compare expression level of two or more metatranscriptomic samples?
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You could use last to generate these results quickly, using some threshold to determine whether you have the same gene fragment.