How to retrive 5' flanking regions (upto 500 bp) from BioMart?

Question

[Chong Tang]

Do you know how to retrieve 5' flanking region from BioMart? I find a similar question in this website. But since Biomart has revised their website. The answer to that question is out of date.

My species is Saccharomyces cerevisiae.

[Manvendra Singh]

do you want co-ordinates or sequences of 5' flanking regions?

Answer 1

[onuralp]

1. Go to BioMart
2. Ensembl Genes 77 (Choose database)
3. Saccharomyces cerevisiae R-64-1-1 (Choose dataset)
4. You may limit the data mining to genes or regions of your interest using Filters section on the left panel
5. Click the Attributes section on the left panel
6. Choose Sequences on the right
7. Expand SEQUENCES (+)
8. Select flanking options of your interest
9. Hit the Results button on the top left to view your dataset
10. Hit the Go button on the top right to download the sequences (fasta format) to your computer