how to make regulatory network from microarray data

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10.1 years ago

goforitpeehu • 0

India

Hiii, I am a beginner, and doing my masters degree research work. I am currently working on gene expression data for saccharomyces cerevisiae with the help of microarray. I want to make a regulatory network of genes which are present in my data. but I am struck as I don't know how to make network from a microarray gene expression data. I installed the GeneMania app in the cytoscape and didn't get any idea how to proceed.

gene genome • 2.9k views

ADD COMMENT • link updated 2.8 years ago by Ram 44k • written 10.1 years ago by goforitpeehu • 0

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Cytoscape, did you read the manual. It has so many plug-ins. more you explore, more you get into the networks. :)

ADD REPLY • link 10.1 years ago by Manvendra Singh ★ 2.2k

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Personally, I'd not be very confident in such analysis basing on gene expression data alone. It shows only potential changes on the protein level. Tools like GeneMania will find plethora of interactions but it should be interpreted with great caution. @goforitpeehu Try to find some evidence at the protein level (eg. using data from public repositories like, arrayexpress, pride, geo).

ADD REPLY • link 10.1 years ago by boczniak767 ▴ 870

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I am a beginner, and doing my masters degree research work.

Do you want to pinpoint a fundamental flaw in your educational system?

ADD REPLY • link updated 2.8 years ago by Ram 44k • written 10.1 years ago by Michael 55k

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