Mutate A Triplet From A Protein Sequence

Question

[Flow]

Given a protein sequence, is it there any experimental method that allows to detect a given triplet and change or mutate it to another one?

[Neilfws]

If by "experimental method" you mean "in the laboratory", then this is not a bioinformatics question and should be closed. And if you're looking for publications, you should learn how to search literature effectively.

[Gww]

In what organism?

[Larry_Parnell]

Triplet is a confusing word. Are you asking about a codon triplet, or, as I interpret your questions, do you want to know about detecting a 3-amino acid sequence within the larger polypeptide? Both can be detected and can be mutated.

[Flow]

yes Larry, exactly that. I would like to get the most representative references or publications about that, thanks

[Flow]

I tried it but found nothing; which keywords would you use? codon + triplet + mutate ?

[Zhidkov]

What you are looking for called "Site-directed mutagenesis".

Answer 1

[Omid]

I think you can do it by site directed mutagenesis