how to read gene expression RNA seq

Question

[Am.A]

I am familiar with gene expression (microarray data) now I want to explore gene expression of RNA-seq

What are numbers means? Is there a site that I can download gene expression for RNA seq?

what is the difference between gene expression for mircoarray and RNA-seq?

Answer 1

[Manvendra Singh]

I think you need to read some reviews, they would be very very useful, because in one sentence or in one post, can't describe everything

Here are some useful links, I think others would also suggest some nice literature

• http://www.nature.com/nrg/journal/v10/n1/full/nrg2484.html
• http://www.ncbi.nlm.nih.gov/pubmed/21627854
• http://bioinformatics.oxfordjournals.org/content/25/12/1476.full
• http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4152252/ and this one
• http://www.sciencemag.org/content/320/5881/1344

Answer 2

[Ahill]

I'd also recommend looking at SEQC's recent paper which does an extensive comparison of microarray and RNASeq: http://www.nature.com/nbt/journal/v32/n9/full/nbt.2957.html

As for sites where you can access RNA-Seq data, SRA would be one place: http://www.ncbi.nlm.nih.gov/sra

[Am.A]

Thank you for your reply Could you please describe more how to download RNA seq gene expression data?

[HuzH]

You can get data frrom NCBI or ENCODE, just filter by RNA-seq. https://www.encodeproject.org/matrix/?type=Experiment&status=released