Question

Mirna Primer Designing

2

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13.7 years ago

Adarsh Kuamr ▴ 60

Hello

How can we design primer for mature miRNA for RT-PCR quantification?

Thank you

primer mirna • 8.9k views

ADD COMMENT • link updated 2.4 years ago by Ram 45k • written 13.7 years ago by Adarsh Kuamr ▴ 60

score 1 · Answer 1 · 2011-10-31

1

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13.5 years ago

Michael 55k

You can't! miRNA is too short to get a sufficient primer pair.

ADD COMMENT • link 13.5 years ago by Michael 55k

Ram · Answer 2 · 2015-06-28

0

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9.9 years ago

Wubin Qu ▴ 170

ADD COMMENT • link updated 2.4 years ago by Ram 45k • written 9.9 years ago by Wubin Qu ▴ 170