about blast2go problem

Question

[Kurban]

hello.
i am using blast2go to annotate my sequences(which contain 30000 sequences), last night it annotated fine, but in the morning because of the network connection abnormality it is aborted. i tried again since then(tried with the .fa file with 4 sequences), but it is not working and constantly shows this "Negative CloudBlast Computation Units Balance: You have now consumend all CloudBlast computation units available for your subscription." why it does not working?

[GouthamAtla]

This clearly indicates that you have used the free quota allocated to you. You may need to pay for Blast2go PRO.

[Kurban]

Thanks @Geek_y,

Then I can use blast (notcloudblast)to finish my job right? if I could how long time does it take to blast around 30000 sequences?

Answer 1

[Stefan]

From within Blast2GO you have various options to get your Blast+ (NCBI Blast) job done:

• CloudBlast (paid Blast2GO PRO Community Resource Cloud System)
• NCBI Blast (online Blast against the NCBI)
• Local Blast (download the database of your choice and start blasting on you local PC)
• Blast using your own Amazon Cloud Instance.

The speed obviously depends, in case of a local blast, on your database size and number of CPUs. In case of the NCBI blast on the speed/traffic of the NCBI resources. A blastx of 30.000 sequences against the NR database could take you some 10 days at the NCBI, easily.

Tip: Make sure you start the InterProScan at the same time since this step also takes quite a while. They can be done in parallel very nicely in Blast2GO.

Once you have these results the functional annotation process should be relatively quick.

[sgoetz123]

All these Blast2GO features and more are now available via OmicsBox from https://www.biobam.com/omicsbox/