Nucleotide sequence of an Enzyme

Question

[pradeep.pant25]

Hi,

I want to download nucleotide sequence of all hydrolases and Isomerases enzyme found in humans. Please suggest me how to download them all at a time.

[a.polo88]

http://www.uniprot.org/uniprot/?query=hydrolases&sort=score

in this case you have all hydrolases on the left you can apply the selection humans (353 sequences) and download it.

[pradeep.pant25]

Thanks for reply, but this will give me amino acid sequence of those 353 sequences and I am asking for nucleic acid sequence.

Answer 1

[a.polo88]

hi, on uniprotkb you can search for hydrolases and isomerases sequences then you can selected the species in this case human and select all and click on download.

example :

http://www.uniprot.org/uniprot/?query=hydrolases&fil=organism%3A%22Homo+sapiens+%28Human%29+[9606]%22&sort=score

[pradeep.pant25]

your method will give me amino acid sequence only but not nucleotide sequence of those enzymes and I am looking for nucleotide sequence of enzymes.

thanks anyway.

Answer 2

[Pierre Lindenbaum]

search GO for hydrolase: http://amigo.geneontology.org/amigo/term/GO:0016787

download the genes associated to this term: Gene Names From Go Accessions Numbers

then get the sequences: How To Download Gene Sequences From Ncbi Gene

[a.polo88]

it's is true him ask about nucleotide sequences i have seen bad but in this case is not to much simple to use ensembl?

Answer 3

[Michael]

You can try the following Ensembl biomart query.