Hi all, how to convert text file to cel file? I have a dataset in text file format. My role is to find out differentially expressed genes, so I'm trying it in R with limma package and facing many difficulties so please can anybody help me out?
You need to install affypackage from bioconductor in R and then use it to import your data.
For agilent, there is no cel format file. using limma directly. (http://www.bioconductor.org/packages/release/bioc/vignettes/limma/inst/doc/usersguide.pdf)
Yes - the .cel format is only for Affy arrays, and you can only go from a .cel file to a .txt file (not the other way around, because you lose information during the conversion). Also, some Agilent arrays are 2-color arrays, which would make no sense you tried to analyze them like an Affy array.
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version 2.3.6
It's agilent.
It's asking me for cel files or gzipped .. I'm unable to do both.