How to predefine Chipseq peak calling peak length in MACS?
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10.3 years ago
SP ▴ 300

Dear All,

I have been searching for a way to tell MACS that I want to look for narrow or broad peaks. Unfortunately, I haven't figured out which parameters are responsible.

Thanks

ChIP-Seq • 2.1k views
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Entering edit mode
10.3 years ago
TriS ★ 4.8k

I think you can use the --broad parameter when calling MACS2. you can also specify the cutoff using --broad-cutoff

That allows you to put together smaller (close) regions into one broader region.

ref: https://github.com/taoliu/MACS/

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