Best book for (practical) Next Generation Sequencing Bioinformatics?

Question

[Samuel Lampa]

I'm taking a course in NextGen sequeincing bioinformatics in a couple of weeks, and wanted to start out preparing by reading a good book, preferrably with a strong focus on the practical aspects, but something that combines theory and practice is maybe even better.

I would hope to find something like the really best computer programming books, that combine a bit of theory with proper, well explained, hands-on examples.

Also, my background is that I have a M.Sc. in Biotechnology Engineering, but have mostly worked on the computational / infrastructure side of things in the bioinformatics field so far, so while I have a good foundation in biochemistry, biology, bioinformatics and programming / scripting, I feel I have a lot to learn about the specifics within (NGS) sequence analysis.

Looking forward to your recommendations?

(Edit: Of course, if the really best material is something online, please include that too!)

(Edit 2: Added my background)

[Samuel Lampa]

Just want to update here, that after looking around for good practical books for many years, and finally also getting a bit more time to deliberately study sequencing bioinformatics (apart from my own field of pharmaceutical bioinformatics/cheminformatics) , I finally got me a subscription on the Biostar handbook, and the included Applied Bioinformatics course and I'm completely hooked. So extremely to the point, practical and informative. I will not need to look further.

Answer 1

[Johan Hermansson]

Not sure about best or not, but perhaps you'll find https://en.wikibooks.org/wiki/Next_Generation_Sequencing_%28NGS%29 suitable for your needs.

[Daniel]

That's a great resource, although lacking in some areas e.g. the Chromatin page is empty

[Samuel Lampa]

Many thanks, Johan! :)

Answer 2

[rtliu]

RNA-seq Data Analysis: A Practical Approach

You could read the first chapter here

Besides, Applied Bioinformatics 2014 by the BioStars creator is also very practical.

[Samuel Lampa]

Many Thanks!

I definitely had the RNA-Seq book on my radar (and I will most probably get it at some point), as one of the authors is my colleague here at Scilifelab :) The type of "practical approach" book is exactly the type of book I'm looking for! Only I wanted to see if I could find something that covers NGS analysis a little broader than just the RNA-Seq part.

Also, the Applied Bioinformatics course materials looks very interesting!