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9.8 years ago
samuelksm
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0
i annotated my bacterial sequence with basys annotation server the annotation came back with 7500 gene but all the genes appear to be on the positive strand, so i decided to re annotate with RAST this time 4500 genes came back both on positive and negative strands. i wanted to work with the basys file but why are they all on the positive strand, can any one explain to me what is happening?
Perhaps this from the docs: https://www.basys.ca/server1/basys/cgi/howto.pl
Thanks Albert, i contacted the Basys annotation server workers and they just replied the server contained a bug, but they had fixed so i just have to re annotate my sequences.