Entering edit mode
9.8 years ago
biotech
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570
I would like to extract selected genomic region containing a protein we are studying and 1-2 Kb up- and down-stream.
I thought performing alignment against reference genome and parsing the MAF file would be feasible.
Could you suggest me some tools to perform this task?
Thanks!
I'm also considering some alternatives to perform this task, such as BLASTn Extract fasta from BLAST hits
What exactly are you trying to align against the genome? A single sequence, a large dataset, something else entirely?
200 bacterial draft genomes of the same specie.