why are all the genes on the positive strand after annotation using Basys annotation server?
0
0
Entering edit mode
9.8 years ago
samuelksm • 0

i annotated my bacterial sequence with basys annotation server the annotation came back with 7500 gene but all the genes appear to be on the positive strand, so i decided to re annotate with RAST this time 4500 genes came back both on positive and negative strands. i wanted to work with the basys file but why are they all on the positive strand, can any one explain to me what is happening?

sequencing • 2.4k views
ADD COMMENT
0
Entering edit mode

Perhaps this from the docs: https://www.basys.ca/server1/basys/cgi/howto.pl

The defline must be in the form >identifier:cstart-end name, where: specifies that the coding sequence is on the complementary strand. If the coding sequence is on the direct strand, just omit the c character.

ADD REPLY
0
Entering edit mode

Thanks Albert, i contacted the Basys annotation server workers and they just replied the server contained a bug, but they had fixed so i just have to re annotate my sequences.

ADD REPLY

Login before adding your answer.

Traffic: 1313 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6