cluster comparison-gene diagrams

Question

[samuelksm]

Hello,

i have been trying to map and compare gene clusters like this .http://openi.nlm.nih.gov/imgs/512/194/2686493/2686493_gkn751f6.png i have tried using clc work bench, snap gene, fancy gene but non comes close to that diagram in the link. so i have three newly sequenced strains and i want to compare one cluster against P.sp. f113. any ideas on how to go about this problem or even softwares. By the way, my scripting is still novice.

Answer 1

[dago]

Not exactly like in the link but these software could help you:

• http://easyfig.sourceforge.net/
• http://multigeneblast.sourceforge.net/

[samuelksm]

Dago thank you for your reply, i have tried using the easyfig but i cannot get the diagram i want, i only get a black line, i figured it could be the input gbk file, i have fasta files and i have tried to convert them to gbk format but they wont generate a proper diagram using easyfig. i converted the fasta files to gbk using emboss tools.so how can i generate a proper genebank file?