Hello
I intend to use Infinium/Illumina 450K human methylation array data in cancer studies. I need to understand if data for normal sample is required? I am planning to download data from tcga (where normal data isn't available).
Also another basic doubt is "if the normal and tumor sample is mixed when loading onto the slide"?
For studying methylation changes in a disease, comparison to "normal" is required. Is that right?
Also to find the differentially methylated regions, "normal" or "control" must be required. Please correct me if I am thinking in a wrong direction.
What is the biological question?
I get your point. Thank you.