Are these steps correct? Galaxy
1
0
Entering edit mode
9.9 years ago
rus2dil ▴ 20

I have aligned NGS six runs of a sample using BWA (galaxy platform). Then I merged them into one BAM file. Next I sliced the region of interest using SliceBAM in BED tools. After that I merged sequences in the resulting file using Merge command in Operate on Genomic Intervals. So the final result is a BED file. How can I convert this BED file into a FASTA file?

alignment next-gen-sequencing Assembly • 1.9k views
ADD COMMENT
1
Entering edit mode
9.9 years ago

I don't think you can do it in Galaxy but you can convert with bedtools getfasta

ADD COMMENT

Login before adding your answer.

Traffic: 1996 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6