Individual VCF files from main VCF file

Question

[win]

Hi all,

In the 1000 genomes project there is one large VCF file which has all the samples represented in columns.

I want to generate one VCF file for each sample, how can this be done.

Also with the script that can do this, is it possible to stream the main VCF so that I don't have to store it locally.

Thanks in advance

Answer 1

[Jorge Amigo]

already stated here and here:

for file in *.vcf*; do
  for sample in `bcftools query -l $file`; do
    bcftools view -c1 -Oz -s $sample -o ${file/.vcf*/.$sample.vcf.gz} $file
  done
done

Answer 2

[Pierre Lindenbaum]

I wrote Biostar130456 https://github.com/lindenb/jvarkit/wiki/Biostar130456

$   curl -sL "https://raw.githubusercontent.com/arq5x/bedtools2/bc2f97d565c36a82c1a0b12f570fed4398001e5f/test/map/test.vcf" |\
    java -jar dist/biostar130456.jar -x -z -p "sample.__SAMPLE__.vcf.gz" 
sample.NA00003.vcf.gz
sample.NA00001.vcf.gz
sample.NA00002.vcf.gz

$ gunzip -c sample.NA00003.vcf.gz
(...)
##source=myImputationProgramV3.1
#CHROM  POS ID  REF ALT QUAL    FILTER  INFO    FORMAT  NA00003
chr1    10  rs6054257   G   A   29  PASS    AF=0.5;DB;DP=14;H2;NS=3 GT:DP:GQ:HQ 1/1:5:43
chr1    20  rs6040355   A   G,T 67  PASS    AA=T;AF=0.333,0.667;DB;DP=10;NS=2   GT:DP:GQ 2/2:4:35
chr1    130 microsat1   GTC G,GTCT  50  PASS    AA=G;DP=9;NS=3  GT:DP:GQ    1/1:3:40
chr2    130 microsat1   GTC G,GTCT  50  PASS    AA=G;DP=9;NS=3  GT:DP:GQ    1/1:3:40

[c7750]

Does this code create only one VCF file with a specific sample ID or create one VCF file per sample in original file?

[Pierre Lindenbaum]

it creates one VCF file per sample in original file

Answer 3

[Lee Katz]

You could do something with the new bcftools v1.1 like this:

bcftools query -H pooled.vcf.gz -f '%CHROM\t%POS\t%REF\t%ALT[\t%SAMPLE=%GT]\n' --samples 'favoriteSample'

I don't think it keeps all the headers but it will give you the information you might want. If you don't want any headers at all, you can remove the -H.

[win]

I wanted each sample to have its own VCF

[Lee Katz]

The --samples argument lets you choose one sample at a time. So you'd have to run this command once per sample.

Answer 4

[GouthamAtla]

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