Trinotate - Error in xls Annotation Report creation
1
0
Entering edit mode
9.8 years ago
oliver.tills ▴ 10

Hi, I am running Trinotate-2.0.1 and have encountered an error in the xls file created.

If I filter by gene_ID in excel it becomes evident that data other than gene ID is in the gene ID column. It turns out that for 10 rows there is an error whereby data are offset, so that the SwissProt_BlastP column takes the place of gene ID and everything else is shuffled around.

My question is - is this a problem with the program, or have I inadvertently done something wrong? The error isn't fixed by deleting the annotation, excel or sqlite files and running again?

Here is a screenshot of the issue - https://www.dropbox.com/s/0dcp2kgvl3mmcb4/Screen%20Shot%202015-02-13%20at%2012.08.56.png?dl=0

Cheers,
Oli

Trinotate • 3.2k views
ADD COMMENT
0
Entering edit mode

Does trinonate actually export an xls file or a tsv/csv file? And are you using different systems in the process (exported in Mac, opened in PC or the other way around)?

ADD REPLY
0
Entering edit mode

No apparently not!

Seems to be a tab delimited file, despite the xls extension. Thanks for asking the question about file formats, I should have twigged this.

ADD REPLY
0
Entering edit mode

So, maybe they're using a mix of vertical \v and regular \t tabs.

ADD REPLY
0
Entering edit mode
9.8 years ago
pld 5.1k

The .xls files I have from trinotate open correctly, did you alter the file at all or change the delimiters in excel when you first opened it? All I can think of is if you said that fields are enclosed in backtick characters, which are used to delimit values within a field.

It should be tab-delimited with LF (\n) to delimit the ends of rows.

There are some bugs in Trinotate however, some of the entries under BLAST hits in results I've seen are truncated. Or there might be some extra characters in the names/symbols/descriptions of various hits.

It does look like some of the EOL characters are screwed up and all of the garbled rows involve Titin. Can you open the file in a text editor (like Notepad++) and display tab/EOL characters and show us the offending sections?

ADD COMMENT

Login before adding your answer.

Traffic: 1691 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6