In steps, how can we predict for protein secretion In gram-positve bacteria, considering the difficulty In uploading protein sequence with Input>100 size?

Question

[heshamgibriel]

PSORTb, and Secretomep are very difficult to use when It comes to uploading data with proteins sequnce up to 100 size, so how can we predict for full proteome secretion using protein predictors?

[dago]

There are plenty of software out there. Many are listed here: http://en.wikipedia.org/wiki/Protein_subcellular_localization_prediction. In case you have more seq then the limit, I think that it is fastest to split the original fasta

[dago]

If I am not mistaken https://rostlab.org/services/loctree2/ does not have limit

Answer 1

[heshamgibriel]

Thanks a lot, I will try to use It and see how It goes.