Predicting functional effects of a mutation

Question

[jan]

I would like to know how to predict the functional effect of a SNP/ mutation given only the codon number and the mismatch.

For example, I only have this information:

gene:p53; codon 244: GGC to AGC, Gly to Ser

Answer 1

[Czh3]

First, your should know the version of the gene annotation used, and than, calculate the chromosome coordinates of this SNP. AND try this:

SIFT

Polyphen2

[jan]

How do I calculate the chromosome coordinates based on the codon number ? all the introns were taken excluded so I can't just simply use the codon number . Any idea ?