Determination of non-clonal/quasi-species from samples

Question

[skbrimer]

Hi Everyone,

I have a broad question, I've been working with segmented dsRNA viral genomes for reovirus and I have noticed interesting alignments using DNAstar's Ngen. For some of the samples I have been working with I have areas where it looks like I have two different species. Its the same for both strands so I do not think its a heterozygosity issuse. I have found a few papers about quasi-species analysis, but I'm not sure I understand them full yet. I need to read them a little closer and a few more times.

So my question is how to I determine if there is a quasi-species in the sample or not and how to I show this to people who may not understand what I'm talking?