how to find different properties of similar proteins ?
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9.7 years ago
Mo ▴ 920

In fact, I just read a news in http://www.sciencedaily.com/releases/2013/06/130627142557.htm

I would like to know how can I search for example for such proteins or find them?

Lets say, I find one, then how can I search family members (those which are similar or have similar functions), should I simply use blast or is there a better way to find them?

I am looking to find the easiest way and if you have any idea, please do not hesitate to let me know

protein • 1.6k views
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9.7 years ago
dago ★ 2.8k

I think there are many approaches you can follow. This also depends from the dataset you are working with.

The first thing would be to take a reference protein sequence and use it as query for a blast search against your dataset/genome.

You can then take the hits and use them as query for a second round fo blast search in you dataset. However, this second step might led to a high number of false positive so you may want to check the hits manually.

A second approach is to take a representative number of sequences for the protein you are interested in for public db. You align them, create a Hidden Markov model and use this model to scan your dataset.

I would recommend you to always double check manually your hits.

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@dago thanks but is there any example? paper? or something like this already published?

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