Resequencing region selection

Question

[smilefreak]

Hi All,

I have been given some data from a candidate gene resequencing experiment. I have recently noticed that processed transcipts are included in the regions. As far as i understand a processed transcript is not thought to code for protein.
Does any one think this could have any downstream affects on analyses such as burden testing.

Thanks for you time.

Answer 1

[Sean Davis]

If you are sequencing DNA, I don't know that protein-coding vs. not is really a major issue in terms of the process of sequencing and calling variants. Determining the functional significance of such variants will be impacted by local annotation (protein-coding vs. not, for example).