gene expression profiling

Question

[bioinfo]

After getting the hub genes or bio marker genes through re analysis of the available GEO data sets by bioinformatics tools, what all further analysis are needed to verify the current results???

Answer 1

[Devon Ryan]

Your post can be rephrased, "I've made a model, how do I test its accuracy?"

The general options for testing the accuracy of a model are:

1. Use datasets not used for model creation to see how predictive/accurate it is.
2. Do a bench experiment where you perturb something important in the model and check if the actual and theoretical results match.

Of course, you might want to check the literature too to ensure that your model is biologically reasonable.

[bioinfo]

Hi... I am finding it difficult to understand yr reply.. Can yu please explain me in an easier way

[Devon Ryan]

What part don't you understand?

[bioinfo]

Regarding model !!! As i havent made any model... i jus used the existing bioinformatics tools to analyse the GEO datasets and i got the hub genes!!!!

[Devon Ryan]

You've constructed a network. That network is a model.

[bioinfo]

Thanks... Can you please give yr email id... i am having so many doubts in gene expression profiling.

[Devon Ryan]

Just post your questions to the site.

[bioinfo]

okie... thanks!!!