Local Pairwise alignment Blast

Question

[farshidshirafkan]

How can I do Local Pairwise alignment Blast for 2 following sequences through NCBI:

1- RBP

2-B-Lactoglobulin

Then do it by lower Gap Penalties ?

[Ram]

What is the context? This looks suspiciously like a designed question (for interviews or assignments or exams), unfortunately.

Answer 1

[Spitshine]

Obtain the sequences as DNA or protein as you require it.

Go to the NCBI website http://1.usa.gov/1yIW9fq

The gap-open and extension penalties can be changed in the "Alignment parameters" section, which you can expand below the submit ("BLAST") button.