identifying of functionally characterized genes homologs by blast and keyword searches against an EST database

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9.6 years ago

zizigolu ★ 4.3k

Hello

I am going to do an extensive BLAST and keyword searches of the citrus transcriptome to identify homologs of the genes involved in steroid biosynthesis.

May someone please give me some tips in this field if possible.

Thank you

blast • 1.8k views

ADD COMMENT • link updated 2.4 years ago by Ram 44k • written 9.6 years ago by zizigolu ★ 4.3k

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Are you looking for similarity to known Citrus genes, or to steroid biosynthesis genes from any organism?

ADD REPLY • link updated 2.4 years ago by Ram 44k • written 9.6 years ago by Neilfws 49k

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I am going to find some absent components in sterol pathway in Citrus based on already characterized gene from model system, but I don't know how do this exactly. If possible give me some clue

Thanks

ADD REPLY • link updated 2.4 years ago by Ram 44k • written 9.6 years ago by zizigolu ★ 4.3k

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