Question

Whole genomic amplification samples for whole exome sequencing

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9.7 years ago

897598644 ▴ 100

Excuse me :

I want to sequence some samples with tiny amount of DNA. Could we use the whole genomic amplification samples? I am concerned that there were already fragments in the amplification samples. I was wondering if I could get enough high-quality DNA fragments after shearing DNA.

Many thanks in advance!

genome next-gen-sequencing • 1.8k views

ADD COMMENT • link updated 2.5 years ago by Ram 44k • written 9.7 years ago by 897598644 ▴ 100

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Is your problem a small amount of DNA, or poor DNA quality, or both?

ADD REPLY • link 9.7 years ago by Sean Davis 27k

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Sorry that I did make it clear. It is a problem of a small amount of DNA.

ADD REPLY • link updated 2.5 years ago by Ram 44k • written 9.7 years ago by 897598644 ▴ 100

score 0 · Answer 1 · 2016-04-04

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8.7 years ago

jianggl2000 ▴ 50

you may look into this paper, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3883664/

ADD COMMENT • link 8.7 years ago by jianggl2000 ▴ 50