Analysis of 454 sequences of honey bee pathogens

Question

[ironya2000]

I am learning bioinformatics; I have 454 sequences which I dont know how to interpret/understand/analyse, please help.

I used RNA from adult bees and brood to detect the pathogens circulating in honey bee colonies, used GS FLX.

How do I post an example of my sequence?

[PoGibas]

Can you edit post with example of your data?

Answer 1

[Daniel]

You haven't said what type of data/experiment you've done, but I think you might have microbial amplicon seq. If that's the case you should check out qiime: http://qiime.org/tutorials/tutorial.html

[ironya2000]

I extracted RNA from bee brood and adult bees, did cDNA sythesis using superscript II and converted cDNA to DsDNA using Klenow reaction, then did amplification, GS FLX Titanium sequencing, got resultant sequences.

[Adrian Pelin]

Then the simplest/quickest thing to do is to map your 454 reads to known honeybee parasite genomes sequenced to date.

[ironya2000]

Thanks, sorry for bothering you, this is a grey area to me, please assist me on how to do mapping.

since there are more that one pathogen identified, how do I pick the refernce sequences?