how to validate the predicted N-linked glycosylation site based on amino acid information?

Question

[p.thejkiran]

Trying to use only sequence based information to validate apart from the motif for N-linked glycosylation. I could think of Topology of the protein sequence, but still looking for many other ways to validate

Thanks in advance

Answer 1

[shelly.deforte]

Aside from the motif, you could also look at general homology to proteins with known N-linked glycosylation sites. If it were a different type of PTM, I might suggest looking at intrinsic disorder or a structural motif, but there doesn't seem to be a preference for structure or disorder in N-linked glycosylation. My only other thought would be to look at common interaction partners. Not just the oligosaccharyltransferases, but maybe some other proteins that provide some fine tuning? Sounds like a fun project!

[p.thejkiran]

Thanks for your inputs