How to annotate the nucleotide sequences in terms of KEGG Orthology (KO)?

Question

[bdpunith]

I have a RNA-Seq data in which I have to construct the KEGG Pathway for DGE between two samples. I also have nucleotide sequences (contigs) in the fasta format (.fa) file. Kindly tell me how to assign the KO IDs to these nucleotide sequences. Also help me in construction of pathways either using KEGG, Cytoscape or any other pathway network builder software/server.

Answer 1

[dago]

Try BLASTKOALA or KEGGMAPPER

Answer 2

[Naren]

kaas automatic server can do that. Check here.