Promoter Motifs (+) and (-) strands

Question

[rus2dil]

Hello,

I have been researching on putative motif on a promoter region. PLACE (http://www.dna.affrc.go.jp/PLACE/index.html) signal scan gave me results like this.

 (+) = Current Strand
 (-) = Opposite Strand


1      TGTGCCCACCACAGCACACCTATCGTTATCATCAGCGTGGACTAAGAACA
                          (-)GATAMOTIF S000039
                                (-)GATAMOTIF S000039

51     GAACAATTTCTCATCTTTTGTTTCCTGAGAAGTTTAGACCACCAAGATAT
         (+)CAATBOX1 S000028
                                                   (+)GATAMOTIF S000039

101    ATGCTGATGATGCTGTAGATAGCTAGCTAGTTTTGCAATTACTTATGGGT
                       (+)GATAMOTIF S000039
                                         (+)CAATBOX1 S000028
                                                   (-)SEF3MOTIF S000115

151    TGCATCACAATCAGGGTCTGTTTAGTTCCCAAACAAAATTTTTCACGCTG
             (+)CAATBOX1 S000028
                                           (-)-300ELEMENT S000122

201    TTACATAGGATGTTTGGACACATGCATAGAGTACTAAATGTAGAAAAAAA
                        (+)EBOXBNNAPA S000144
                        (-)EBOXBNNAPA S000144
                          (+)RYREPEAT1 S000100
                          (+)RYREPEAT2 S000105

251    ACAATTAAACATTTCGCCTTGAAATTACGAGACAAATCTTTTAAGCCTAA
       (+)CAATBOX1 S000028
        (+)POLASIG2 S000081

My gene is in (+) strand and motifs will be mapped. So should I consider motifs (-) strand too? Do (-) strand motifs affect a gene in (+) strand?

Answer 1

[mark.ziemann]

Yes you should consider motifs on the minus strand to be as important as those on the plus strand. There is a large body of research on bidirectional promoters.

[rus2dil]

Thank you very much for the information. I will look into that