smallRNA-seq, but have files R1/R1fastq?
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Entering edit mode
9.5 years ago
manekineko ▴ 150

Hi all,

I had files from small RNA-seq (single end), but when I see in the archive I got R1-R2 files, I thought these are specific to pair-end? So I'm confused a bit?

ACGTG_L008_R1_001.fastq
ACGTG_L008_R1_002.fastq
ACGTG_L008_R1_003.fastq
ACGTG_L008_R1_004.fastq
ACGTG_L008_R1_005.fastq
ACGTG_L008_R2_001.fastq
ACGTG_L008_R2_002.fastq
ACGTG_L008_R2_003.fastq
ACGTG_L008_R2_004.fastq
ACGTG_L008_R2_005.fastq
fastq smallRNAs single-end • 1.9k views
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I think you should best talk to your sequencing provider. What do the R2 files contain?

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